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DEVELOPMENT OF FIELD METHODOLOGY TO RAPIDLY DETECT DEHALOCOCCOIDES AND DEHALOBACTER SPP. GENES ON-SITE
Cupples, A.M., S.A. Hashsham, R.D. Stedtfeld, and P.B. Hatzinger.
SERDP Project ER-2309, 223 pp, 2019

Loop-mediated isothermal amplification (LAMP) is a novel molecular method recently developed for the specific detection of nucleic acids. LAMP is a one-step amplification reaction that amplifies a target DNA sequence using four to six primers. The Bst large-fragment DNA polymerase has strand displacement activity and helicase-like activity that allow it to unwind and amplify DNA strands in the 60-65°C temperature range. Because LAMP is rapid, sensitive, and specific and occurs isothermally, it has emerged as an alternative to real-time quantitative PCR-based methods in a wide variety of applications. Further, because LAMP does not require a real-time thermal cycler (amplification is isothermal), the method allows less expensive and potentially field-deployable detection devices to be used. https://www.serdp-estcp.org/content/download/48829/465375/file/ER-2309%20Final%20Report.pdf See also the Executive Summary at https://www.serdp-estcp.org/content/download/49113/468390/file/ER-2309%20Executive%20Summary.pdf.



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