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THE USE OF ADVANCED MOLECULAR BIOLOGICAL TOOLS IN GROUNDWATER CONTAMINATED WITH CHLORINATED SOLVENTS
Cupples, A., M. Michalsen, and K. Kucharzyk.
SERDP & ESTCP Webinar Series, Webinar #97, September 2019

SERDP and ESTCP sponsored two presentations on projects that aimed to measure biomarkers as a mechanism to monitor reductive dechlorination in groundwater contaminated with chlorinated solvents. The first presentation discusses two alternative methods to quantitative PCR-loop mediated isothermal amplification and shotgun sequencing with both taxonomic and functional analyses to measure Dehalococcoides mccartyi genes in groundwater samples that encode for distinct reductive dehalogenases. The second presentation discusses a method to quantify Dehalococcoides reductive dehalogenase proteins as a measure of reductive dechlorination and correlates their abundance with in situ reductive dechlorination rates. https://www.serdp-estcp.org/Tools-and-Training/Webinar-Series/09-05-2019



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