| This report was produced by the U.S. EPA Office of Research and Development. It is designed for technical staff in the EPA Regions and in state agencies that require information on the contribution of Dehalococcoides bacteria to MNA of chlorinated solvents, and information on the proper application and interpretation of the assays in an evaluation of MNA. This report includes sections on the role of biotransformation in evaluation of MNA of chlorinated solvents, the ecology of microorganisms that transform chlorinated solvents, tools to assay microorganisms that transform chlorinated solvents, the relationship between Dehalococcoides DNA in ground water and rates of natural attenuation at field scale, the relationship between geochemical parameters and the occurrence of Dehalococcoides DNA in ground water, and the relationship Dehalococcoides DNA in ground water and behavior of chlorinated solvents in laboratory treatability studies or microcosm studies done with water from the plume. |