Additional Information

Enzyme Activity Probe and Geochemical Assessment for Potential Aerobic Cometabolism of Trichloroethene in Groundwater of the Northwest Plume, Paducah Gaseous Diffusion Plant, Kentucky
Office of Environmental Management
DOE, WSRC-STI-2008-00309, 88 pp, 2008

Field Push-Pull Test Protocol for Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbons
Kim, Y., M. Azizian, J. Istok, and L. Semprini.
Environmental Security Technology Certification Program, 83 pp, 2005

This protocol describes a single-well push-pull test for evaluating the feasibility of using in situ aerobic cometabolic processes to treat groundwater contaminated with chlorinated solvent mixtures.

IRP Aerobic Cometabolic In Situ Bioremediation Technology Guidance Manual and Screening Software User's Guide
Earth Technology Corp., Alexandria, VA Report No: AFRL-ML-TY-TR-1998-4530. NTIS Order No: ADA359333/XAB. 84 pp, June 1998

This document presents the principles of the process, mathematical models used to describe the aerobic cometabolic in situ bioremediation technology, and a discussion of its applicability and limitations. A description is also provided of a software program that can help determine if this technology is appropriate for implementation. The technology was implemented in a full-scale evaluation at Edwards AFB, California. The document includes a discussion surrounding regulatory acceptance of the technology and a description of other field implementations. The report is designed for use by project managers who are exploring potential technology alternatives for groundwater treatment under the Installation Restoration Program (IRP).

Push-Pull Tests for Evaluating the Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbons: ESTCP Cost and Performance Report
Environmental Security Technology Certification Program, NTIS: ADA468544, 46 pp, 2006

Single-well push/pull test methods were demonstrated at Fort Lewis Logistics Center (using toluene as a cometabolic growth substrate) and McClellan AFB (during cometabolic air sparging with propane as a growth substrate) to determine (1) the transport characteristics of nutrients, substrates, and CAHs and their transformation products; (2) the capability of indigenous microorganisms to utilize selected substrates and transform targeted contaminants and surrogate compounds; (3) the rates of substrate utilization and contaminant transformation; and (4) the combinations of injected nutrients and substrates that maximize rates of contaminant transformation.

Standardized Procedures for Use of Nucleic Acid-Based Tools: Recommendations for Groundwater Sampling and Analysis Using qPCR
Lebron, C., P. Dennis, C. Acheson, N. Barros, D. Major, E. Petrovskis, F. Loeffler, K. Ritalahti, C. Yeager, E. Edwards, J. Hatt, and D. Ogles. SERDP Project ER-1561, 12 pp, 2014

SERDP project ER-1561 focused on identifying and minimizing the causes of variability during quantitative real-time polymerase chain reaction (qPCR) enumeration of genes of interest in groundwater, with the goal of developing of the knowledge needed to standardize methods for collecting, preserving, transporting, storing, and processing environmental samples for qPCR analysis. This document summarizes the project conclusions and recommends procedures for using qPCR analyses that will provide data of sufficient accuracy and reproducibility to allow site management decisions regarding bioremediation or MNA. Further details are available in the ER-1561 Final Report (Lebron et al. 2014, 220 pages).

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