Search Result
Technology Innovation News Survey Feed (RSS)
RATIOMETRIC GAS REPORTING: A NON-DISRUPTIVE APPROACH TO MONITOR GENE EXPRESSION IN SOILS
Cheng, H.-Y., C.A. Masiello, I. Del Valle, X. Gao, G.N. Bennett, and J.J. Silberg.
ACS Synthetic Biology [Published online 24 Jan 2018 prior to print]
A new ratiometric gas reporting method is described for non-disruptively monitoring gene expression within hard-to-image environmental matrices. With this approach, ethylene is continuously synthesized by ethylene-forming enzyme to provide information on viable cell number, and methyl bromide is conditionally synthesized by placing a methyl halide transferase gene under the control of a conditional promoter. Ratiometric gas reporting enables the creation of Escherichia coli biosensors that report on acylhomoserine lactone (AHL) autoinducers used for quorum sensing by gram-negative bacteria. The biosensors can be used in soil for non-disruptive monitoring of AHLs synthesized by Rhizobium leguminosarum and degraded by Bacillus thuringiensis. This new reporting approach can also be used in Shewanella oneidensis, a bacterium that lives in sediments. See additional information in the Rice University news release at http://news.rice.edu/2018/02/01/two-stage-gas-sensor-reports-on-soil-dynamics/ .
ACS Synthetic Biology [Published online 24 Jan 2018 prior to print]
A new ratiometric gas reporting method is described for non-disruptively monitoring gene expression within hard-to-image environmental matrices. With this approach, ethylene is continuously synthesized by ethylene-forming enzyme to provide information on viable cell number, and methyl bromide is conditionally synthesized by placing a methyl halide transferase gene under the control of a conditional promoter. Ratiometric gas reporting enables the creation of Escherichia coli biosensors that report on acylhomoserine lactone (AHL) autoinducers used for quorum sensing by gram-negative bacteria. The biosensors can be used in soil for non-disruptive monitoring of AHLs synthesized by Rhizobium leguminosarum and degraded by Bacillus thuringiensis. This new reporting approach can also be used in Shewanella oneidensis, a bacterium that lives in sediments. See additional information in the Rice University news release at http://news.rice.edu/2018/02/01/two-stage-gas-sensor-reports-on-soil-dyn
The Technology Innovation News Survey welcomes your comments and
suggestions, as well as information about errors for correction. Please
contact Michael Adam of the U.S. EPA Office of Superfund Remediation
and Technology Innovation at adam.michael@epa.gov or (703) 603-9915
with any comments, suggestions, or corrections.
Mention of non-EPA documents, presentations, or papers does not constitute a U.S. EPA endorsement of their contents, only an acknowledgment that they exist and may be relevant to the Technology Innovation News Survey audience.
