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VALIDATION OF BIOTECHNOLOGY FOR QUANTIFYING THE ABUNDANCE AND ACTIVITY OF VINYL-CHLORIDE OXIDIZERS IN CONTAMINATED GROUNDWATER: GUIDANCE DOCUMENT
Mattes, T.
ESTCP Project ER-201425, 40 pp, 2018

The purpose of this project was to evaluate qPCR-based molecular diagnostic tools for the purpose of estimating the attenuation contribution of VC-oxidizing bacteria. Groundwater and aquifer samples were taken from several DoD sites. The method targeted functional genes used by etheneotrophic bacteria in the aerobic VC biodegradation pathway. Functional genes associated with both VC oxidation and VC reduction were found present and expressed in groundwater samples. The researchers determined this by analyzing the relationships between functional genes associated with VC biodegradation and geochemical parameters, as well as the bulk VC attenuation rate at these contaminated sites. This novel technology promises to reveal the abundance and functionality of etheneotrophs at VC-contaminated sites. When this information is provided alongside a site-wide VC degradation rate, it could provide evidence that aerobic VC biodegradation is a significant contributor to overall VC natural attenuation processes. https://www.serdp-estcp.org/content/download/47715/454864/file/ER-201425%20Guidance%20Document.pdf



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