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APPLICATION OF MOLECULAR BIOLOGICAL TOOLS FOR MONITORING EFFICIENCY OF TRICHLOROETHYLENE REMEDIATION
Wu, Y.-J., P.-W. G. Liu, Y.-S. Hsu, L.-M. Whang, T.-F.-Lin, W.-N. Hung, and K.-C. Cho.
Chemosphere 233:697-704(2019)

Five pilot-scale wells were constructed to monitor TCE remediation activities in contaminated groundwater, measure the bacterial concentrations and activity after EOS® injections, and correlate biological and gene concentration with TCE degradation. TCE was degraded 42-93% by the end of 175 days due to both EOS® injections and dilution of the aquifer movement among injection and monitoring wells. A Dehalococcoides sp. concentration of 108 copies/L caused the initial 40% degradation of TCE in groundwater. The well with optimal TCE degradation measured 109 cells/L of the tceA gene and had the highest Shannon index number. Desulfovibrio desulfuricans and Desulfuromonas chloroethenica were measured to be the predominant species within the groundwater post-injection. A variety of different factors, including well locations, geochemical indicators, and microbial contribution, were useful to explain the site-specific optimal TCE remediation approach.



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