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ASSESSMENT OF BIODEGRADATION IN NATURAL ATTENUATION PROCESS OF CHLORINATED HYDROCARBONS CONTAMINATED SITE: AN ANAEROBIC MICROCOSM STUDY
Zhang, X., X. Zhu, R. Chen, S. Deng, R. Yu, and T. Long.
Soil and Sediment Contamination: An International Journal 29(2):165-179(2019)
In situ anaerobic microcosm systems were constructed using chlorinated hydrocarbon (CHC)-contaminated soil and groundwater collected from a former pesticide manufacturing site to evaluate natural attenuation potential and its relationships with the geochemistry conditions and microbial communities. Redox conditions measured in both soil and groundwater favored CHC biodegradation processes. Biodegradation was confirmed by comparing PCE and 1,2-DCA degradations along with Cl- accumulation rates between the unsterilized microcosms and the sterilized controls. Using 16S rRNA and Shannon-Winner index analysis revealed distinct shifts of microbial community compositions in the soil and the groundwater microcosm systems during incubation. The dominant species mainly associated with phylum Proteobacteria, Firmicutes, and Actinobacteria. Geobacter-, Streptomyces-, and Acetoanaerobium-associated gene sequences confirmed the in situ biological dechlorination potentials of CHCs. The study showed that an in situ natural attenuation approach was a suitable remediation technique for the CHC-contaminated site.
Soil and Sediment Contamination: An International Journal 29(2):165-179(2019)
In situ anaerobic microcosm systems were constructed using chlorinated hydrocarbon (CHC)-contaminated soil and groundwater collected from a former pesticide manufacturing site to evaluate natural attenuation potential and its relationships with the geochemistry conditions and microbial communities. Redox conditions measured in both soil and groundwater favored CHC biodegradation processes. Biodegradation was confirmed by comparing PCE and 1,2-DCA degradations along with Cl- accumulation rates between the unsterilized microcosms and the sterilized controls. Using 16S rRNA and Shannon-Winner index analysis revealed distinct shifts of microbial community compositions in the soil and the groundwater microcosm systems during incubation. The dominant species mainly associated with phylum Proteobacteria, Firmicutes, and Actinobacteria. Geobacter-, Streptomyces-, and Acetoanaerobium-associated gene sequences confirmed the in situ biological dechlorination potentials of CHCs. The study showed that an in situ natural attenuation approach was a suitable remediation technique for the CHC-contaminated site.
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