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THE DEVELOPMENT OF ANAEROBIC BIOREMEDIATION APPROACHES FOR CHLORINATED SOLVENT AND 1,4-DIOXANE CO-CONTAMINATED SITES
Cupples, A. SERDP Project ER-2712, 85 pp, 2019
The objective of this project was to determine the susceptibility of 1,4-dioxane to biodegradation over a range of redox conditions using multiple inocula sources (uncontaminated agricultural soils, river sediments, and sediments from two 1,4-dioxane contaminated sites) and electron acceptor amendments (iron/EDTA/humic acid, sulfate, and nitrate). Compound specific isotope analysis (CSIA) was used to investigate biodegradation in a subset of the microcosms. Also, DNA was extracted from microcosms exhibiting 1,4-dioxane biodegradation for microbial community analysis using 16S rRNA gene amplicon and shotgun sequencing. The study indicated that 1,4-dioxane biodegradation under anaerobic conditions is feasible. Therefore, natural attenuation of 1,4-dioxane may occur over a wider range of conditions, notably methanogenic areas where highly reducing conditions exist either naturally or as a result of enhanced reductive dechlorination. https://www.serdp-estcp.org/content/download/50677/498041/file/ER-2712%20Final%20Report.pdf
The objective of this project was to determine the susceptibility of 1,4-dioxane to biodegradation over a range of redox conditions using multiple inocula sources (uncontaminated agricultural soils, river sediments, and sediments from two 1,4-dioxane contaminated sites) and electron acceptor amendments (iron/EDTA/humic acid, sulfate, and nitrate). Compound specific isotope analysis (CSIA) was used to investigate biodegradation in a subset of the microcosms. Also, DNA was extracted from microcosms exhibiting 1,4-dioxane biodegradation for microbial community analysis using 16S rRNA gene amplicon and shotgun sequencing. The study indicated that 1,4-dioxane biodegradation under anaerobic conditions is feasible. Therefore, natural attenuation of 1,4-dioxane may occur over a wider range of conditions, notably methanogenic areas where highly reducing conditions exist either naturally or as a result of enhanced reductive dechlorination. https://www.serdp-estcp.org/content/download/50677/498041/file/ER-2712%2
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