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IDENTIFICATION OF A CHLORODIBENZO-P-DIOXIN DECHLORINATING DEHALOCOCCOIDES MCCARTYI BY STABLE ISOTOPE PROBING
Dam, H.T., W. Sun, L. McGuinness, L.J. Kerkhof, and M.M. Haeggblom.
Environmental Science & Technology 53(24):14409-14419(2019)
A DNA-stable isotope probing (SIP) approach identified bacteria active in dechlorinating PCDDs in river sediments using 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TeCDD) as a model. Pyrosequencing of reverse-transcribed 16S rRNA of TeCDD dechlorinating enrichment cultures was used to reveal active members of the bacterial community. Analysis of bacterial community profiles of 13C labeled heavy DNA fraction revealed that an operational taxonomic unit corresponding to Dehalococcoides mccartyi accounted for a significantly greater abundance in cultures amended with 1,2,3,4-TeCDD than in unamended cultures. Results imply that the D. mccartyi strain is involved in 1,2,3,4-TeCDD reductive dechlorination and suggests that SIP can assess the bioremediation potential of organohalogen contaminated sites.
Environmental Science & Technology 53(24):14409-14419(2019)
A DNA-stable isotope probing (SIP) approach identified bacteria active in dechlorinating PCDDs in river sediments using 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TeCDD) as a model. Pyrosequencing of reverse-transcribed 16S rRNA of TeCDD dechlorinating enrichment cultures was used to reveal active members of the bacterial community. Analysis of bacterial community profiles of 13C labeled heavy DNA fraction revealed that an operational taxonomic unit corresponding to Dehalococcoides mccartyi accounted for a significantly greater abundance in cultures amended with 1,2,3,4-TeCDD than in unamended cultures. Results imply that the D. mccartyi strain is involved in 1,2,3,4-TeCDD reductive dechlorination and suggests that SIP can assess the bioremediation potential of organohalogen contaminated sites.
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