U.S. EPA Contaminated Site Cleanup Information (CLU-IN)

U.S. Environmental Protection Agency
U.S. EPA Technology Innovation and Field Services Division


Cometabolic Aerobic and Anaerobic Bioremediation


Adobe PDF LogoEngineered Approaches to In Situ Bioremediation of Chlorinated Solvents: Fundamentals and Field Applications
EPA 542-R-00-008, 2000

This report provides an overview of the fundamentals and field applications of in situ bioremediation to remediate chlorinated solvents in contaminated soil and groundwater. The report describes how chlorinated solvents are degraded, how to enhance the process by the addition of various materials and chemicals, design configurations, and the typical steps taken to evaluate technology feasibility at a specific site. It also includes a list of technology vendors and nine case studies of field applications.

Adobe PDF LogoIRP Aerobic Cometabolic In Situ Bioremediation Technology Guidance Manual and Screening Software User's Guide
Earth Technology Corporation
AFCEE, AFRL-ML-TY-TR-1998-4530, 84 pp, 1998

Provides site screening criteria for the use of cometabolic bioremediation, particularly in groundwater contamination scenarios and presents details on choosing a substrate, formulas for determining the appropriate amount of substrate required, as well as the general principles of the cometabolic remediation process, mathematical models used to describe the technology, and a discussion of its applicability and limitations.

Adobe PDF LogoStandardized Procedures for Use of Nucleic Acid-Based Tools: Recommendations for Groundwater Sampling and Analysis Using qPCR
Lebron, C., P. Dennis, C. Acheson, N. Barros, D. Major, E. Petrovskis, F. Loeffler, K. Ritalahti, C. Yeager, E. Edwards, J. Hatt, and D. Ogles. SERDP Project ER-1561, 12 pp, 2014

SERDP project ER-1561 focused on identifying and minimizing the causes of variability during quantitative real-time polymerase chain reaction (qPCR) enumeration of genes of interest in groundwater, with the goal of developing of the knowledge needed to standardize methods for collecting, preserving, transporting, storing, and processing environmental samples for qPCR analysis. This document summarizes the project conclusions and recommends procedures for using qPCR analyses that will provide data of sufficient accuracy and reproducibility to allow site management decisions regarding bioremediation or MNA. Further details are available in the ER-1561 Final ReportAdobe PDF Logo (Lebron et al. 2014, 220 pages).

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